CCK-8 Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
DNA Ligation:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Protease Inhibitor:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Purification:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
RNA Extraction:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Reverse Transcription:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
SYBR Green Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Real-time Polymerase Chain Reaction:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Labeling:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Staining:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro-transcribed circILNb elicits superior antitumor immunity in cold tumors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FTY720 Sigma-Aldrich Cat#162359-56-0 DNase I SYNTHGENE Cat#10211 RNase R Novoprotein Cat#GMP-E224-U025 Agarose ABclonal Cat#RM02852 Urea Beyotime Cat#ST1731 Safe Red Nucleic acid Dye ABclonal Cat#RM19009 CFSE TargetMol Cat#T6802 SM-102 AVT Cat#O02010 DSPC AVT Cat#S01005 Cholesterol AVT Cat#O01001 DMG-PEG2000 AVT Cat#O02005 Fluc mRNA (5 ′ CAP) GLBIO Cat#GM10002 CCK-8 Beyotime Cat#C0037 IL-2 TargetMol Cat#TMPY-02788 GM-CSF zen-bioscience Cat#PD201239 IL-4 zen-bioscience Cat#PD201184 IFN-γ zen-bioscience Cat#PD201231 PD-1 R and D Systems Cat#1021-PD-100 N-803 MedChemExpress Cat#HY-P99661 Percoll Solarbio Cat#P8370 Brefeldin A BioLegend Cat#420601 D-Luciferin BD Biosciences Cat#556877 Penicillin-streptomycin solution BioChannel Biological Technology Cat#BC-CE-007 Critical commercial assays DNA Ligation Mix ABclonal Cat#RK21501 Cusa Fast Pfu DNA Polymerase CUSABIO Cat#CSB005A T7 High Yield RNA Transcription Kit New England Biolabs Cat#E2050S Safe Red Nucleic acid Dye ABclonal Cat#RM19009 Lipofectamine Messenger Max Invitrogen Cat#MAN0010803 Protease inhibitor cocktail NJJCBIO Cat#W060-1-1 Hypersensitive ECL substrate zen-bioscienc Cat#17046 SPARKeasy/PCR Purification Kit Shandong Sparkjade Biotechnology Cat#AE0101-C RNA Extraction Kit ABclonal Cat#RK30120 Reverse Transcriptase Kit ABclonal Cat#RK21400 SYBR Green qPCR Mix ABclonal Cat#RK21203 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Lightning Link® R-PE Labeling Kit Abcam Cat#ab102918 Dual-Luciferase Assay kit Promega Cat#E1960 Quant-iT RiboGreen RNA Assay Kit Thermoscientific Cat#R11490 BCA kit Life-iLab Cat#AP12L025 Mycoplasma test kit HUABIO Cat#K0103 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat#554714 BD Phosflow Fixation Buffer I BD Biosciences Cat#557885 BD Phosflow Fixation Buffer III BD Biosciences Cat#558050 IL-15 ELISA Kit Wuhan Fine Biotech Cat#EM1156 ALT ELISA Kit Jingmei Biotechnology Cat#JM-02543M2 (Continued on next page) Cell Reports Medicine 6, 102413, October 21, 2025 e2 OPEN ACCESS ..
Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
Recombinant:Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
Enzyme-linked Immunospot:Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
H&E Stain:Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
TUNEL Assay:Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
Apoptosis Assay:Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER DynaSpatial FFPE Spatial Gene Expression Slides Dynamic Biosystems Cat#13020031 Deposited data scRNA-seq/Target-seq/Spatial transcriptomics This paper NGDC: CRA022987 https://ngdc. cncb.ac.cn/gsa/browse/CRA022987 TCGA LIHC dataset GDC Data Portal TCGA: LIHC https://portal.gdc.cancer. gov/projects/TCGA-LIHC GSE156625 dataset Gene Expression Omnibus GEO: GSE156625 https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE156625 Spatial transcriptome dataset from Liu’s research Mendeley Data http://www.doi.org/10.17632/skrx2fz79n.1, https://data.mendeley.com/datasets/ skrx2fz79n/1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 DC2.4 Merck Cat#SCC142 Hepa 1-6 ATCC Cat#CRL-1830 LLC ATCC Cat#CRL-1642 Experimental models: Organisms/strains C57BL/6 mouse (male, 6–8 week old) Slac Laboratory Animal N/A Recombinant DNA PUC57-eGFP This paper N/A PUC57-Neoantigens This paper N/A PUC57-OVA This paper N/A Software and algorithms ImageJ v2.17.0 National Institutes of Health (NIH) https://imagej.net/software/fiji/ Living Image 4.2 PerkinElmer https://www.perkinelmer.com Fusion Software v2.2.0 (PhenoCyclerFusion acquisition) Akoya Biosciences https://www.akoyabio.com/phenocycler/ inForm Tissue Analysis Software v3.0.0 Akoya Biosciences https://www.akoyabio.com/phenoimager/ inform-tissue-analysis-software/ GraphPad Prism 8.0 GraphPad software https://www.graphpad.com/ Flow Jo 10.0 BD Bioscience https://www.flowjo.com/ R v4.3.3 R Foundation https://www.r-project.org/ Celescope v1.17.0 Singleron https://github.com/singleron-RD/CeleScope Seurat v5.1.0 Rahul Satija https://satijalab.org/seurat/ Harmony v1.2.1 Ilya Korsunsky https://portals.broadinstitute.org/harmony/ SingleR v2.4.1 SingleR-inc https://github.com/SingleR-inc/SingleR SCEVAN v1.0.1 Antonio De Falco https://github.com/AntonioDeFalco/SCEVAN CellChat v2.1.2 Cellchat https://github.com/jinworks/CellChat ClusterProfiler v4.10.1 YuLab-SMU https://github.com/YuLab-SMU/clusterProfiler DynamicST v1.0.4 Dynamic Biosystems https://github.com/DynamicBiosystems/ DynamicST Stlearn v0.4.1 Duy Pham https://stlearn.readthedocs.io/en/latest/ TESLA v1.2.4 Jian Hu https://github.com/jianhuupenn/TESLA Other Fluc-mRNA TriLink Cat#L-7202 RNase R Beyotime Cat#R7092M RiboGreen RNA Reagent Shanghai Maokang Cat#MF0785 Red blood cell lysis Solarbio Cat#R1010 mm10 reference genome Gencode https://www.gencodegenes.org/mouse/ e3 Cell Reports Medicine 7, 102754, May 19, 2026 OPEN ACCESS
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